With Kuroageha's powers sealed, the Ten Crucifix Insects of Sanada, a group of powerful mushi-hybrid warriors advance from the west killing everyone at their path. After consulting with Nagatomimaru, the Mushi Magistrate departs to Kishuu to look for a way to regain her powers, accompanied only by Jinbei and one of the Shogun's most trusted retainers - Ooka Echizen.